epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Product Overview

The ChromaFlash Chromatin Extraction Bundle is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle. The isolated chromatin will even be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is acceptable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

Background Information
Chromatin immunoprecipitaton (ChIP) offers an advantageous machine for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions may very well be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may very well be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Principle & Course of
The ChromaFlash™ Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation may very well be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous machine for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions may very well be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may very well be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Bundle accommodates all the reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

ExoDNA? Extraction Kit

K1242-25
EUR 705.6

ExoDNA? Extraction Kit

K1242-50
EUR 1057.2

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08

Nuclear Protein Extraction Kit

BSP009 50Preps
EUR 106.98

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

EpiQuik Nuclear Extraction Kit

OP-0002 100 Assays
EUR 350.58
Description: kits suitable for this type of research

Membrane Protein Extraction Kit

K2113-50 50 assays
EUR 652.8

Mammalian Cell Extraction Kit

K2114-500 500 assays
EUR 385.2

Membrane Protein Extraction Kit

K268-50
EUR 646.8

Plant Tissue Extraction Kit

K296-50
EUR 548.4

Leave a Comment

Your email address will not be published.