HRP Conjugated Arachis

HRP Conjugated Arachis

Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker  it strongly recognises essentially the most cancers explicit T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an extra specificity within the path of Galbeta1–>4GlcNAc (LacNAc), which is not tumour explicit, had been attributed to PNA.

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, fairly than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and completely different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is generally used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is out there in a stabilized liquid sort.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply. It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily applicable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over completely different detection methods comprises quite a few exposures, blots may be reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market approach.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an applicable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one factor peroxidase substrate (SKU: 21530073).

Software program

Frequent Western Blot Protocol:

  • Glycoprotein sample dimension: 500ng
  • Lectin Focus: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS net web page gel
  3. Swap gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/Physiol Actions

PNA does not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very like the anti-T antibody in human sera. The lectin might be utilized to inform aside between human lymphocyte subsets.
arachis eylabs
arachis eylabs

Unit Definition

One unit will sort 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily sort

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast approach, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-01mg 0.1mg
EUR 350

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-05mg 0.5mg
EUR 505

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-1mg 1mg
EUR 895

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-5x1mg 5x1mg
EUR 3885

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS614645-1mL 1mL
EUR 565

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS614645-5x1mL 5x1mL
EUR 2395

Arachis hypogaea Conglutin-7

1-CSB-EP757666ANE
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli

Arachis hypogaea lectin (PNA)

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Arachis hypogaea lectin (PNA

05-0116-1000 1 g Ask for price

Arachis hypogaea lectin (PNA

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Arachis hypogaea 2S protein

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Arachis hypogaea 2S protein

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Arachis hypogaea 2S protein

MBS717441-5x10mg 5x10mg
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rAra h 1 (Arachis hypogaea 1.0101)

10-P202-C100 0.1mg
EUR 242

rAra h 2 (Arachis hypogaea 2.0201)

10-P208-C100 0.1mg
EUR 242

rAra h 8 (Arachis hypogaea 8.0101)

10-P210-C100 0.1mg
EUR 242

rAra h 9 (Arachis hypogaea 9.0101)

10-P232-C100 0.1mg
EUR 242

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