Genomic Epidemiology of Historical Clostridium perfringens Outbreaks in New York State Using Two Web-based Platforms: National Center for Biotechnology Information-Pathogen Detection and FDA-GalaxyTrakr

Clostridium Perfringens is the second cause of bacterial bacterial diseases in the United States. The center of Wadsworth (WC) in the New York Health Department of the State calculates the dose of infection from primary patients and food samples and to date, identifying C. perfringens only at the species level. We investigate whether the order-based subtyping of all genomes can benefit the epidemiological investigation of these pathogens, as well as other enteric organisms. We retrospectively sort 76 patients’ samples and food received between May 2010-February 2020, including 52 samples associated with epidemiologists up to 13 places and 24 sporadic samples were not related to other samples.

Filogenetic trees are built using two web-based platforms; The National Center for Information on Pathogen Detection Biotechnology (NCBI-PD) and GalaxyTrakr (Galaxy Instance is supported by the genometrakr initiative). For the analysis of the GalaxyTrakr, a single nucleotide polymorphism matrix (SNP) and the maximum possible tree (mL) resulting using 3 different reference genomes. In four separate analyzes, phylogenetic grouping is generally in accordance with epidemiological outbreaks. The diversity of SNP among samples in philogenically related to the outbreak ranges from 0-20 SNP, except for one outbreak from 4-62 SNP. Important, four of the 13 playpes harbored one or more samples that were phylogenetic outliers, and for two outbreaks, no samples were closely related. Three specimens found to store two different genotypes.

For the sample below the threshold of the CDC enumeration dose, the grouping of phylogenetics is strong and related to patient samples and / or food. We conclude that the WGS phylogenetic cluster is: 1) mostly in accordance with epidemiological outbreaks, regardless of the analysis platform or the reference genome we use; 2) Having paired SNP diversity in pairs, allows phylogenetic clusters to be distinguished from sporadic cases; 3) can help in epidemiological investigations by identifying outlier and polyclonal samples.

Integrate research into molecular cloning courses to deal with developing biotechnology landscapes

The rapid development of molecular biotechnology presents curricular challenges for educators who try to provide students with relevant courses. Comprehensive biological education must also include opportunities for students to develop intellectual and technical skills through authentic research experience. However, integrating relevant and interesting research projects into their classes, however, can be a challenging task for instructors. To overcome these various requests, we redesign our existing molecular cloning courses to include independent research projects that assess calcium signaling. In the revised course, students use traditional and recombination-based cloning strategies to produce vector expression of bacteria and mammals encoded by Campari, a novel calcium indicator.

Campari expressed bacterially used in protein tests and purification tests. Students also have to design their own research project that evaluates the effects of chemotherapy agents on calcium signaling in the mammalian system. The revised and novel laboratories are designed to be modular, facilitate their integration to 2 years of course. End semester student evaluation is compared between years that reveal significant differences in student perceptions of the difficulty of the course between years. Changes in this attitude highlight the potential of pedagogical considerations that must be examined when introducing new materials and activities into existing courses.

Biotechnology for the World Tomorrow: Scenario to guide the direction for future innovation

Depending on how the future will be revealed, today’s progress in biotechnology research has greater or smaller potential to be the basis of further innovation. Tracking progress on indicators for a different future scenario will help to focus, emphasize, or emphasize research on timely discovery and to maximize opportunities for successful innovation. In this paper, we show how learning scenarios with 2050 horizons help recognize the implications of political and social development in the potential for ongoing biotechnology research innovations. We also propose a model to further enhance open innovation between academics and biotechnology values ​​to help fundamental research exploring the field of inventions that have greater opportunities for applied research.

Genome Skimming and NMR Chemical Fingerprinting provides quality assurance biotechnology to validate the identity and purity of Sarsaparilla

Sarsaparilla is a popular natural health product (NHP) that has been reported as one of the most common botany in the market. Some reasonable explanations are documented including economicly motivated product substitutions, unintentional errors because of ambiguous trade names related to several different taxa, and wild harvesting from the wrong non-commercial factory.

Unfortunately, this includes the case of the species of Adulteran Decalepis HamiltonII, the red drug plant species are registered by the International Union for Nature Conservation (IUCN) and declared a species with high conservation concerns by the Indian National Biodiversity Authority (NBA). This study provides a validated genomic biotechnology solution (NMR Chemical Fingerprints) to prevent adultery in raw materials and finished products. It is also the first use of Oxford Nanopore on herbal products that allow the use of the skimming genome as a tool for quality assurance in the supply chain of botanical materials.

T-Pro Plasmid Midi Kit (20)
RB94-YPI020	T-Pro Biotechnology	20preps/Kit	EUR 255.6

T-Pro Plasmid Maxi Kit (10)
RB94-YPM010	T-Pro Biotechnology	10preps/Kit	EUR 266.4

T-Pro Genomic DNA Midi Kit (20)
RB94-NGM020	T-Pro Biotechnology	20preps/kit	EUR 266.4

T-Pro Genomic DNA Mini Kit (100)
RB94-NGS100	T-Pro Biotechnology	100preps/kit	EUR 255.6

T-Pro Endotoxin Removal Plasmid Midi kit (25)
RB94-EPI020	T-Pro Biotechnology	20preps/Kit	EUR 297.6

T-Pro Endotoxin Removal Plasmid Maxi kit (10)
RB94-EPM010	T-Pro Biotechnology	10preps/Kit	EUR 318

T-Pro Plant Genomic DNA Midi Kit (20)
RB94-PGM020	T-Pro Biotechnology	20preps/kit	EUR 266.4

T-Pro Plant Genomic DNA Mini Kit (100)
RB94-PGS100	T-Pro Biotechnology	100preps/kit	EUR 266.4

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002M	T-Pro Biotechnology	250ml*2/Kit	EUR 244.8

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002S	T-Pro Biotechnology	100ml*2/Kit	EUR 182.4

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004M	T-Pro Biotechnology	250ml*2/Kit	EUR 286.8

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004S	T-Pro Biotechnology	100ml*2/Kit	EUR 204

T-Pro EZ Gel Solution 8%
JB02-B008M	T-Pro Biotechnology	500ml/BT	EUR 266.4

T-Pro EZ Gel Solution 8%
JB02-B008S	T-Pro Biotechnology	100ml/BT	EUR 162

T-Pro EZ Gel Solution 10%
JB02-B010M	T-Pro Biotechnology	500ml/BT	EUR 266.4

T-Pro EZ Gel Solution 10%
JB02-B010S	T-Pro Biotechnology	100ml/BT	EUR 162

T-Pro EZ Gel Solution 12%
JB02-B012M	T-Pro Biotechnology	500ml/BT	EUR 266.4

T-Pro EZ Gel Solution 12%
JB02-B012S	T-Pro Biotechnology	100ml/BT	EUR 162

T-Pro EZ Gel Solution 15%
JB02-B015M	T-Pro Biotechnology	500ml/BT	EUR 266.4

T-Pro EZ Gel Solution 15%
JB02-B015S	T-Pro Biotechnology	100ml/BT	EUR 162

T-Pro Separating or Resolving Buffer
JB03-C001	T-Pro Biotechnology	500ml/BT	EUR 182.4

T-Pro Transfer Blotting buffer (10X)
JB08-H001	T-Pro Biotechnology	500ml/BT	EUR 162

T-Pro Western Blot Stripping Reagent
JB11-K002	T-Pro Biotechnology	500ml/BT	EUR 213.6

T-Pro Aqua EZ Clean (M)
JT90-R001M	T-Pro Biotechnology	500ml/BT	EUR 398.4

T-Pro Aqua EZ Clean (S)
JT90-R001S	T-Pro Biotechnology	100ml/BT	EUR 181.2

T-Pro EZ stain Gel solution
JT90-R005M	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro EZ stain Gel solution
JT90-R005S	T-Pro Biotechnology	100ml/BT	EUR 151.2

T-Pro P-Fect Transfection Reagent
JT97-N005M	T-Pro Biotechnology	1.0ml/vial	EUR 266.4

T-Pro Protein Free Blocking Buffer (in PBS)
JK92-W003	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)
JK92-W004	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro Gel/PCR DNA Purification Maxi Kit (10)
RB94-NEL010	T-Pro Biotechnology	10preps/Kit	EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (100)
RB94-NES100	T-Pro Biotechnology	100preps/Kit	EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)
RB94-NES250	T-Pro Biotechnology	250preps/Kit	EUR 266.4

T-Pro Fast Blocking Buffer (in PBS)
JK92-W001	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)
JK92-W002	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro Laemmli (SDS sample) Reagent (reducing 4X)
JB06-F002	T-Pro Biotechnology	10ml/BT	EUR 151.2

T-Pro Tris-Glycine-SDS running buffer (10X)
JB07-G001	T-Pro Biotechnology	500ml/BT	EUR 162

T-Pro Tris-Glycine-Native running buffer (10X)
JB07-G002	T-Pro Biotechnology	500ml/BT	EUR 162

T-Pro Semi Dry Transfer Blotting buffer (10X)
JB08-H002	T-Pro Biotechnology	500ml/BT	EUR 172.8

T-Pro Nonliposomal Transfection Reagent I (NTR I)
JT97-N001M	T-Pro Biotechnology	1.0ml/vial	EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)
JT97-N002M	T-Pro Biotechnology	1.0ml/vial	EUR 244.8

T-Pro Nonliposomal Transfection Reagent III (NTR III)
JT97-N006M	T-Pro Biotechnology	1.0ml/vial	EUR 266.4

Bradford Protein Assay Kit
abx090644-1000tests	Abbexa	1000 tests	EUR 247.2

Bradford Protein Assay Kit
abx097194-500Assays	Abbexa	500 Assays	EUR 303.6

Bradford Protein Assay Kit
abx098125-100ml	Abbexa	100 ml	EUR 266.4

Bradford Protein Assay Kit
SK3031	Bio Basic	1000Assays, 1000preps	EUR 89.23

Bradford Protein Assay Kit
Z5030028	Biochain	500 assays	EUR 410.4
Description: At some point almost every protein study comes to the Bradford assay. A rountine step that, if wrongly performed, can destroy your entire experiment. Be safe and order a premade kit that guarantees sucessful outcome.

T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)
JB06-F003	T-Pro Biotechnology	10ml/BT	EUR 151.2

T-Pro Washing buffer in PBS and Tween-20 (10X)
JB09-I003	T-Pro Biotechnology	500ml/BT	EUR 172.8

T-Pro Washing buffer in TBS and Tween-20 (10X)
JB09-I004	T-Pro Biotechnology	500ml/BT	EUR 172.8

T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing
JB09-I001	T-Pro Biotechnology	500ml/BT	EUR 162

T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing
JB09-I002	T-Pro Biotechnology	500ml/BT	EUR 162

Coomassie (Bradford) Protein Assay Kit
AR0145	BosterBio	1kit	EUR 84

Bradford Assay Kit, 1,000 tests
209	AthenaES	Kit	EUR 220.8

BSA Calibrator [Bradford Assay Kit]
0209C	AthenaES	0.5 ml	EUR 123.6

Better Bradford Protein Assay Kit (Improved linearity)
SK3041	Bio Basic	1000Assays, 1000preps	EUR 99.67

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050	GenDepot	450ml	EUR 225.6

Amplite® Colorimetric Bradford Protein Quantitation Assay Kit
11118	AAT Bioquest	1000 Tests	EUR 102

Amplite® Colorimetric Bradford Protein Quantitation Assay Kit
11119	AAT Bioquest	5000 Tests	EUR 211

Bradford(Coomassie) Protein Assay Plus Kit, BSA 1mlx 5
P7200-050	GenDepot	Kit	EUR 254.4

Bradford Protein Quantification Kit
E111-01	Vazyme	500 rxn	EUR 158.4

Bradford Protein Quantification Kit
E111-02	Vazyme	1000 rxn	EUR 188.4

Renilla Luciferase Assay Kit 2.0
30082-T	Biotium	50AS	EUR 136.8
Description: Minimum order quantity: 1 unit of 50AS

Firefly Luciferase Assay Kit 2.0
30085-T	Biotium	50AS	EUR 130.8
Description: Minimum order quantity: 1 unit of 50AS

Bradford reagent
BDE641	Bio Basic	100ml	EUR 73.21

Firefly & Renilla Luciferase Single Tube Assay Kit
30081-T	Biotium	50AS	EUR 186
Description: Minimum order quantity: 1 unit of 50AS

Detergent Compatible Bradford Protein Quantification Kit
E211-01	Vazyme	500 rxn	EUR 213.6

NucView 488 CASPASE 3 ASSAY KIT, TRIAL SIZE
30029-T	Biotium	1ST	EUR 182.4
Description: Minimum order quantity: 1 unit of 1ST

Bradford Dye Reagent
0209R	AthenaES	100 ml	EUR 157.2

ATP-Glo Bioluminometric Cell Viability Assay Kit (50 assays)
30020-T	Biotium	1KIT	EUR 102
Description: Minimum order quantity: 1 unit of 1KIT

Cdc42 Activation Assay Kit, Trial Size
STA-402-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

Ral Activation Assay Kit, Trial Size
STA-408-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay Kit, Trial Size
STA-409-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Testosterone (T) ELISA Kit
DLR-T-Ge-48T	DL Develop	48T	EUR 562.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids.

Testosterone (T) ELISA Kit
DLR-T-Ge-96T	DL Develop	96T	EUR 729.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids.

Steady-Luc Firefly HTS Assay Kit (trial size)(4 mL)
30028-T	Biotium	1KIT	EUR 112.8
Description: Minimum order quantity: 1 unit of 1KIT

Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays)
30002-T	Biotium	1KIT	EUR 219.6
Description: Minimum order quantity: 1 unit of 1KIT

Pan-Ras Activation Assay Kit, Trial Size
STA-400-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

General Testosterone (T) ELISA Kit
RD-T-Ge-48Tests	Reddot Biotech	48 Tests	EUR 560.4

General Testosterone (T) ELISA Kit
RD-T-Ge-96Tests	Reddot Biotech	96 Tests	EUR 775.2

General Testosterone (T) ELISA Kit
RDR-T-Ge-48Tests	Reddot Biotech	48 Tests	EUR 585.6

General Testosterone (T) ELISA Kit
RDR-T-Ge-96Tests	Reddot Biotech	96 Tests	EUR 811.2

Albumin Standard (2.0 mg/ml)
JB04-D001C	T-Pro Biotechnology	5*1ml/kit	EUR 162

OxiSelect Protein Carbonyl Fluorometric Assay, Trial Size
STA-307-T	Cell Biolabs	20 assays	EUR 421.2
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content.

OxiSelect TBARS Assay Kit (MDA Quantitation), Trial Size
STA-330-T	Cell Biolabs	20 assays	EUR 408
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect Catalase Activity Assay Kit, Fluorometric, Trial Size
STA-339-T	Cell Biolabs	50 assays	EUR 477.6
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

OxiSelect Catalase Activity Assay Kit (Colorimetric), Trial Size
STA-341-T	Cell Biolabs	20 assays	EUR 435.6
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kits measure catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 100 assays in a 96-well microtiter plate, or 50 assays in microcentrifuge tubes. This includes blanks, catalase standards and unknown samples. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The OxiSelect Catalase Activity Assay Kit (Colorimetric) utilizes visible light (520 nm), which reduces sample interference. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

Rac1 Activation Assay Kit, Trial Size
STA-401-1-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay Kit, Trial Size
STA-401-2-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

RhoA Activation Assay Kit, Trial Size
STA-403-A-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay Kit, Trial Size
STA-403-B-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay Kit, Trial Size
STA-403-C-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Rap1 Activation Assay Kit, Trial Size
STA-406-1-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit, Trial Size
STA-406-2-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay Kit, Trial Size
STA-407-1-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay Kit, Trial Size
STA-407-6-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit, Trial Size
STA-312-T	Cell Biolabs	20 assays	EUR 338.4
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size
STA-342-T	Cell Biolabs	20 assays	EUR 414
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Fluorometric), Trial Size
STA-344-T	Cell Biolabs	50 assays	EUR 344.4
Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase.   In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin.  The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm.  Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples.  The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit, Trial Size
STA-360-T	Cell Biolabs	20 assays	EUR 338.4
Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Lipoprotein Lipase (LPL) Activity Assay Kit (Fluorometric), Trial Size
STA-610-T	Cell Biolabs	20 assays	EUR 351.6
Description: The Lipoprotein Lipase (LPL) Activity Assay Kit is a simple, fluorometric assay that quantitatively measures LPL activity in plasma, serum, and lysates in a 96-well microtiter plate format.  Each kit provides sufficient reagents to perform up to 100 assays, including blanks, LPL standards, and unknown samples.  The kit contains a LPL Standard and has a detection sensitivity limit of ~1 mUnits/mL (see Kit Components for unit definition). Besides LPL, this assay can also be used to detect endothelial and hepatic lipase activity.

H-Ras Activation Assay Kit, Trial Size
STA-400-H-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

K-Ras Activation Assay Kit, Trial Size
STA-400-K-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

N-Ras Activation Assay Kit, Trial Size
STA-400-N-T	Cell Biolabs	5 assays	EUR 483.6
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size
STA-347-T	Cell Biolabs	20 assays	EUR 400.8
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect Superoxide Dismutase Activity Assay, Trial Size
STA-340-T	Cell Biolabs	20 assays	EUR 414
Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1016-100-T	Biovision	each	EUR 874.8

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1019-100-T	Biovision	each	EUR 907.2

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1022-100-T	Biovision	each	EUR 888

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1332

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS410A-KIT	SBI	1 kit (10 rxn)	EUR 798

Locking T-Handle
M-CP-111-065-T	MiTeGen	1 UNIT	EUR 55
Description: Locking T-Handle

AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS
AP-STR-KIT-P	CORNING	1/pk	EUR 865.2
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS420A-KIT	SBI	1 kit (10 rxn)	EUR 828

CytoSelect 24-Well Wound Healing Assay, Trial Size
CBA-120-T	Cell Biolabs	6 assays	EUR 428.4
Description: Traditionally scratch assays have been used to study cell migration, cell proliferation and wound healing. However, these assays lack a consistently defined wound gap and can result in high inter-sample variation. Our CytoSelect 24-Well Wound Healing Assay provides a much more consistent method to measure cell migration across a wound field gap in vitro. Proprietary inserts generate a consistent 0.9mm wound gap between the cells. Cells may then be treated or monitored for proliferation or migration across the wound field by imaging samples at fixed time points or by time-lapse microscopy.

ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)
M1007-100-T	Biovision	each	EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)
M1010-100-T	Biovision	each	EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)
M1013-100-T	Biovision	each	EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1025-100-T	Biovision	each	EUR 888

96-Well Cellular Senescence Assay Kit (SA-?-gal Activity, Fluorometric Format), Trial Size
CBA-231-T	Cell Biolabs	24 assays	EUR 414
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

CytoSelect 96-Well Phagocytosis Assay(Zymosan Substrate), Trial Size
CBA-224-T	Cell Biolabs	20 assays	EUR 463.2
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)
M1004-100-T	Biovision	each	EUR 1136.4

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Shikari (S-ATD) Denosumab (Prolia) Qualitative ELISA kit
DEN-QLS-PRO	Matriks Biotek	1x96-wells test plate	EUR 999
Description: Enzyme Immunoassay for Qualitative detection of Denosumab (Prolia) in human serum and plasma samples.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 319.2

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size
CBA-135-T	Cell Biolabs	24 assays	EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

OxiSelect Oxygen Radical Antioxidant Capacity (ORAC) Activity Assay, Trial Size
STA-345-T	Cell Biolabs	48 assays	EUR 379.2
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect Hydroxyl Radical Antioxidant Capacity (HORAC) Activity Assay, Trial Size
STA-346-T	Cell Biolabs	48 assays	EUR 379.2
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

Shikari (Q-DEN) Denosumab (Prolia)Free drug ELISA kit
DEN-FD-PRO	Matriks Biotek	1x96-wells test plate	EUR 1053.6
Description: Enzyme Immunoassay for detection of freeDenosumab (Prolia) in human serum and plasma samples.

Pesticide Kit Containing All 10 Multi-Compound Mixes
SPXPR-KIT	Scientific Laboratory Supplies	1ML	EUR 1923.18

Fmoc-Pro-Pro-Pro-Pro-Pro-OH
B-3660.0050	Bachem	50.0mg	EUR 211.2
Description: Sum Formula: C40H47N5O8; CAS# [454693-94-8]

Fmoc-Pro-Pro-Pro-Pro-Pro-OH
B-3660.0250	Bachem	250.0mg	EUR 733.2
Description: Sum Formula: C40H47N5O8; CAS# [454693-94-8]

CytoSelect 24-Well Cell Migration Assay (8 µm, Colorimetric Format), Trial Size
CBA-100-T	Cell Biolabs	4 assays	EUR 358.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (8 µm, Fluorometric Format), Trial Size
CBA-101-T	Cell Biolabs	4 assays	EUR 358.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (5 µm, Fluorometric Format), Trial Size
CBA-102-T	Cell Biolabs	4 assays	EUR 358.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 24-Well Cell Migration Assay (3 µm, Fluorometric Format), Trial Size
CBA-103-T	Cell Biolabs	4 assays	EUR 358.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 24-Well Cell Invasion Assay (Basement Membrane, Colorimetric Format), Trial Size
CBA-110-T	Cell Biolabs	4 assays	EUR 386.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 24-Well Cell Invasion Assay (Basement Membrane, Fluorometric Format), Trial Size
CBA-111-T	Cell Biolabs	4 assays	EUR 386.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation), Trial Size
CBA-130-T	Cell Biolabs	24 assays	EUR 463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size
CBA-140-T	Cell Biolabs	24 assays	EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

GloMeltâ„¢ Thermal Shift Protein Stability kit
33021-T	Biotium	1kit	EUR 292.8
Description: Minimum order quantity: 1 unit of 1kit

GloMeltâ„¢ Thermal Shift Protein Stability kit
33022-T	Biotium	1kit	EUR 319.2
Description: Minimum order quantity: 1 unit of 1kit

AccuOrangeâ„¢ Protein Quantitation Kit, 200 assays
30071-T	Biotium	1KIT	EUR 121.2
Description: Minimum order quantity: 1 unit of 1KIT

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT	SBI	1 Kit	EUR 1358.4

BCA Protein Assay Kit
20-abx090640	Abbexa	EUR 266.40 EUR 322.80	200 tests 500 tests

BCA Protein Assay Kit
abx098126-100ml	Abbexa	100 ml	EUR 303.6

BCA Protein Assay Kit
20-abx293001	Abbexa	EUR 284.40 EUR 811.20 EUR 435.60	100 tests 2500 tests 500 tests

Protein Carbonyl Assay Kit
abx257881-96tests	Abbexa	96 tests	EUR 764.4

BCA Protein Assay Kit
AR0146	BosterBio	1kit	EUR 121.2

BCA Protein Assay Kit
AR0146-1L	BosterBio	1kit	EUR 547.2

BCA Protein Assay Kit
AR0146-500	BosterBio	1kit	EUR 339.6

BCA Protein Assay Kit
SK3021	Bio Basic	500Assays, 500preps	EUR 127.86

Lowry Protein Assay Kit
SK4031	Bio Basic	1000Assays, 1000preps	EUR 87.14

QuantiChrom Protein Assay Kit
QCPR-500	BioAssay Systems	500	EUR 304.8
Description: Quantitative determination of total protein at 595nm (Bradford method). Procedure: 2 min. Kit size: 500 tests. Detection limit: 60 µg/mL. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

QuantiFluo Protein Assay Kit
QFPR-200	BioAssay Systems	200	EUR 226.8
Description: Quantitative determination of total protein by fluorimetry at 360/450nm (compatible with handheld fluorimeter catalog # FL360450). Procedure: 10 min. Kit size: 200 tests. Detection limit: 50 ng/mL. Shelf life: 6 months. Shipping: RT; storage: -20°C.

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT	SBI	10 rxn	EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT	SBI	10 rxn	EUR 1358.4

Boc-Pro-Pro-Pro-Pro-OH
A-2615.0100	Bachem	100.0mg	EUR 254.4
Description: Sum Formula: C25H38N4O7; CAS# [29804-52-2]

Boc-Pro-Pro-Pro-Pro-OH
A-2615.0250	Bachem	250.0mg	EUR 471.6
Description: Sum Formula: C25H38N4O7; CAS# [29804-52-2]

Fmoc-Pro-Pro-Pro-Pro-OH
B-3680.0050	Bachem	50.0mg	EUR 194.4
Description: Sum Formula: C35H40N4O7; CAS# [454693-96-0]

Fmoc-Pro-Pro-Pro-Pro-OH
B-3680.0250	Bachem	250.0mg	EUR 675.6
Description: Sum Formula: C35H40N4O7; CAS# [454693-96-0]

H-Pro-Pro-Pro-Pro-OH
H-4800.0050	Bachem	50.0mg	EUR 356.4
Description: Sum Formula: C20H30N4O5; CAS# [21866-90-0]

H-Pro-Pro-Pro-Pro-OH
H-4800.0250	Bachem	250.0mg	EUR 1311.6
Description: Sum Formula: C20H30N4O5; CAS# [21866-90-0]

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 966

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT	SBI	1 Kit	EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT	SBI	1 Kit	EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT	SBI	1 Kit	EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT	SBI	1 Kit	EUR 1358.4

[Ile-Ser] - Bradykinin (T - Kinin) [Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg; MW: 1260.47]
SP-88234-5	Alpha Diagnostics	5 mg	EUR 343.2

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT	SBI	10 rxn	EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT	SBI	10 rxn	EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT	SBI	10 rxn	EUR 1358.4

Validation of genomic and metabolomic approach provides a quality assurance perspective for product identity and purity. This study allows the manufacture and retailers to verify their authentic supply chains and that consumers can enjoy safe and healthy products.