Direct PCR

Phire and Phusion Kits

Unit Definition:

One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid-insoluble substance inside 30 min at 74°C.

Description Code Specification Class Expertise
ALS SARS-CoV-2 RT-PCR Take a look at for surfaces ALS-B01-Adv RUO Meals /Water Virology Detection Actual-Time PCR
ALS SARS-CoV-2 RT-PCR Take a look at for Wastewater ALS-B02-Adv RUO Meals /Water Virology Detection Actual-Time PCR

What’s Direct PCR?

Direct PCR (dPCR) is a technique of DNA amplification straight from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This method drastically reduces experimental time, and price in genotyping and high-volume tasks. It additionally supplies a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step may doubtlessly result in pattern loss.

In some ways, dPCR works like typical PCR. Each strategies depend upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow sturdy amplification regardless of the presence of PCR inhibitors typically present in crude samples.

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced solely at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all recognized SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different recognized associated viruses or widespread respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been based mostly on WHO, CDC and DGS suggestions.

The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical gadget (CE-IVD) that makes use of real-time PCR nucleic acid amplification expertise for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who could or is probably not suspected of getting COVID-19.

The equipment makes use of the RT-PCR approach, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment may also detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.

Direct PCR Reagent
Direct PCR Reagent

Benefits

  • Speedy amplification: The amplification charge of the polymerase is 6kb/min. 1kb fragment could be amplified inside 25min.
  • Lengthy fragment amplification: For plasmid, λ DNA and different simple templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
  • Excessive specificity: With sizzling begin expertise, the polymerase is 100% inactive beneath 50°C, and may solely be restored by heating at 95°C for 5min.
  • Excessive tolerance to impurities: Samples of complete blood, serum, cultured cells and urine could be straight amplified with out prior DNA purification.

Unit Definition

One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Plant Direct PCR Kit

PD105-02 200 rxn (50 μl/rxn)
EUR 159.5

Plant Direct PCR Kit

PD105-00-10rxns 10 rxns
EUR 1.09

Plant Direct PCR Kit

PD105-02-200rxns 200 rxns
EUR 165.16

Direct PCR Complete Kit

S700 100 rcs
EUR 159.6

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 628.8

Direct Blood PCR Kit

MBPCR171-100R 1 unit
EUR 267.52
Description: Direct Blood PCR Kit

Direct Blood PCR Kit

MBPCR171-10R 1 unit
EUR 71.73
Description: Direct Blood PCR Kit

Direct Blood PCR Kit

E0950-01 100reactions
EUR 69.76
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Blood PCR Kit

E0950-02 500reactions
EUR 277.95
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Plant PCR Kit

E0960-01 100reactions
EUR 55.59
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

Direct Plant PCR Kit

E0960-02 500reactions
EUR 251.79
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

Direct Tissue PCR Kit

E0940-01 100reactions
EUR 55.59
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Direct Tissue PCR Kit

E0940-02 500reactions
EUR 251.79
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 51.41

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 188.5

Mouse tissue direct PCR kit

EP033-200T 200T
EUR 125

Mouse tissue direct PCR kit

EP033-50T 50T
EUR 40

Mouse tissue direct PCR kit

EP033 50T
EUR 125

Mouse Tissue Direct PCR Kit

RK20736 100RXN
EUR 150

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 165.6

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

BioLit Animal Tissue Direct PCR Kit (Research)

91210 50 Preps
EUR 222.28
Description: Part E

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02100 100 RXNS Ask for price

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02500 500 RXNS Ask for price

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