Biovision Rnase-R

RNase R from BioVision


RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the look at of lariat RNA produced by standard splicing, along with circRNAs which come up by the use of back-splicing. By eradicating linear
RNAs from cell or RNA extracts, RNase R drastically facilitates the identification of spherical species by the use of RNA-sequencing. This allows researchers to probe the panorama of splicing events with increased depth.


• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying numerous splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Steer clear of repeated freeze-thaw cycles of all components to retain most effectivity. All components
are regular for one 12 months from the date of transport when saved and handled appropriately.

Biovision rnase-r
Biovision rnase-r
Cat # +Dimension M1228-500
Dimension 500 U
Highlights RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions -20°C
Supply Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.


• For digestion of full RNA, longer incubations of 2-Three hours are typically required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement additional enzyme partway (e.g. 0.5 μl after 1 hour) by the use of the method. The higher temperature is very useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this may end in non-enzymatic RNA degradation.

• RNase R shows low train on tRNA, rRNA and totally different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use additional RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Do not forget that spherical RNAs symbolize a small proportion of full RNA (typically 0.1%-0.01%), as a consequence of this reality RNase R treatment will most actually finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is helpful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be useful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation will be utilized in its place. For NGS, robust half reversible immobilization (SPRI) bead cleanup is helpful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.


• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Bundle (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

RNase R

M1228-500 each
EUR 438

RNase R

MBS4156446-500Units 500Units
EUR 330

RNase R

MBS4156446-5x500Units 5x500Units
EUR 1245

RNase R (20 U/μl)

RNR-E5146 2KU
EUR 716.9
Description: RNase R (20 U/μl) is expressed from Escherichia coli K12 E. coli.

RNase H (RNase-free)

K1093-1000 1000U
EUR 42
Description: RNase H

RNase H (RNase-free)

K1093-500 500U
EUR 24
Description: RNase H

RNase H (RNase-free)

K1093-5000 5000U
EUR 120
Description: RNase H


E16FXP0211R-200 200 μl
EUR 13.33
Description: Available in various conjugation types.


E16FXP0211R-400 400 μl
EUR 20
Description: Available in various conjugation types.


E16FXP0211R-800 800 μl
EUR 26.67
Description: Available in various conjugation types.


TRI-A40S1 100UL
EUR 37.8

Rnase (90-105)

MBS8247015-10mg 10mg
EUR 715

Rnase (90-105)

MBS8247015-1mg 1mg
EUR 260

Rnase (90-105)

MBS8247015-5mg 5mg
EUR 510

Rnase (90-105)

MBS8247015-5x10mg 5x10mg
EUR 3140

RNase A

abx098139-1ml 1 ml
EUR 260.4

PI / RNAse

abx290029-200tests 200 tests
EUR 326.4

RNase A

MB087-100MG 1 unit
EUR 34.02
Description: RNase A

RNase A

MB087-1G 1 unit
EUR 284.22
Description: RNase A

RNase A

MB087-500MG 1 unit
EUR 153.8
Description: RNase A

RNase A

RM7705-100MG 1 unit
EUR 136.11
Description: RNase A

RNase A

RM7705-1G 1 unit
EUR 1102.26
Description: RNase A

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