BACKGROUND: Modulation of the immune system with a vaccine lymphoma cells genetically modified is a potential therapeutic value in the treatment of B cell lymphoma, however, the anti-tumor effect of single immunogen transfer so far been limited. the combination therapy of recombinant IL-2 and IL-12 have been reported synergistically to stimulate an anti-tumor response in solid tumors, but the potential of IL-2 / IL-12 gene-modified B cell lymphoma cells has not been explored.
METHODS: Using three human B cell lines cell lymphoma distinct and primary samples from patients with neoplasms of B cells, the level of expression of the coxsackie B-adenovirus receptor (CAR) and alpha (v) integrin analyzed by fluorescence-activated sorting of cells (FACS). Adenoviral transduction efficiency is determined by analysis of GFP expression and IL-2 and IL-12 cytokine production was quantified by enzyme-linked immunosorbent assay (ELISA) test. proliferation activity of peripheral blood mononuclear cells (PBMC) stimulated with either cytokine derived from transduced lymphoma cell supernatant was measured by cell proliferation (MTT) test.
EuTDA cytotoxicity assay was used to compare the cytotoxic activity of IL-2 and / or IL-12 stimulated PBMCs against lymphoma cells modified. RESULTS: We found that B cell lymphoma cell lines can be transduced with a much higher efficiency than the primary tumor samples, which appears to correlate with the expression of CAR. increased Adenoviral-expressed IL-2 and IL-12 together cause a dose-dependent proliferation levels of PBMCs obtained from a healthy donor.
IL-2 and / or IL-12 transduced cell lymphoma were co-cultured with PBMCs, which were tested for cytolytic activity against lymphoma cells modified. We found that IL-2 stimulated PBMC anti-tumor effects were significant, but the combined effect of IL-2 / IL-12 or IL-12 alone. CONCLUSIONS: This study suggests that the generation of adenovirus recombinant modified vaccine lymphoma cells based on lines lymphoma cells expressing IL-2 and IL-12 cytokine gene is technically feasible, induces a rise in the level of proliferation and cytotoxic activity of co-cultured PBMC, and warrants further development for the treatment of lymphoma patients in the future.
Effects of recombinant adenovirus-mediated expression of IL-2 and IL-12 in human B lymphoma cells on co-cultured PBMC.
Proteomic analysis of prostate cancer cells resistant zoledronic-acid introduce novel pathways invasive phenotype characteristics.
Proteomic analysis of differentially expressed proteins were identified between zoledronic acid-resistant cells and DU145R80 aggressive prostate cancer (PCa) and their parental DU145 cells. Ingenuity Pathway Analysis (IPA) showed a strong relationship between the protein identified in the network associated with cancer and with a prevalently homogenous cellular functions related to the regulation of cell organization, and movement consistent with smaller and reduce contact cells DU145R80 cell morphology. identified proteins correlated in human PCa published genomic data provided by the increased expression of the tumor and aggressiveness.
DU145R80 also exhibits a clear increase of alpha-v (αv) integrins, and the urokinase receptor (Upar), both included in the same network of proteins identified. Interestingly, local actin-rich structures in the outskirts of cells rich DU145R80 of Filamin A, one of the proteins identified and Upar which, in turn, co-localize with αv-integrin, in podosomes and / or invadopodia.
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. This is a cost efficient bulk pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits to avoid unsealing the plates and reagents that won't be immediately used.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: Integrin alpha5 is a protein encoded by the ITGA5 gene which is approximately 114,5 kDa. Integrin alpha5 is localised to the cell membrane and is involved in the MAPK-Erk pathway, apoptotic pathways, integrin pathway, focal adhesion and blood-brain barrier and immune cell transmigration. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signalling. Integrin alpha5 is expressed in the liver, lung, nervous system, bone marrow and blood. Mutations in the ITGA5 gene may result in colon carcinoma and congenital epulis STJ97284 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Integrin alpha5 protein.
Description: A sandwich ELISA kit for detection of Integrin Alpha 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
Especially, the invasive features DU145R80 can be prevented by blocking anti-αv antibody. Overall, we uncover the signaling network that physically connects the interior of the nucleus through the extracellular matrix to the cytoskeleton and can dictate PCa aggressiveness shows potential novel prognostic markers and therapeutic targets for patients with PCa.
