Make your private pGreenFire 2.zero reporter vector or use as a detrimental administration
With the pGreenFire 2.zero mCMV Cloning & Unfavorable Administration Lentivector & Virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro), you could benefit from our sturdy pGreenFire 2.zero lentivector know-how to create your private transcriptional response part (TRE) reporter or use pGreenFire 2.zero mCMV as-is as a detrimental administration.
With the pGreenFire 2.zero mCMV Cloning & Unfavorable Administration Lentivector, XhoI and NheI web sites are positioned upstream of a minimal CMV promoter (mCMV) so you could clone in your private TREs. Upon activation, the TREs and mCMV promoter collectively drive co-expression of crimson firefly luciferase and GFP so you could quantitatively measure transcriptional train using every fluorescence and luciferase train.
Alternatively, you should use the vector as-is as a detrimental administration for any enterprise using pGreenFire 2.zero lentivectors (see info below).
What makes our next-gen pGreenFire 2.zero vectors even larger than totally different TRE reporter vectors is the great design, which gives in a constitutive selection cassette for regular cell line period whereas minimizing interference with the upstream TRE. By using a weak/cheap mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and totally arranging the conditional reporter genes, the selection marker is reliably expressed with out compromising conditional expression of rFLuc and GFP.
SBI Lentiviral Utilized sciences
NEW PRODUCT ALERT!
SBI is labored as much as announce the launch of our subsequent period of pGreenFire signalling pathway reporters! We’ve upgraded these normal lentivectors with a clever design that allows reliable period of regular cell traces and have moreover modified the standard luciferase reporter with crimson firefly luciferase, which opens up the potential of performing a dual-spectral luciferase assay and delivers bigger sensitivity for in vivo capabilities than typical luciferase.
pGreenFire Fundamentals
For many who’re not acquainted with our pGreenFire reporters, every 1.zero and a few.zero share a similar core efficiency—transcriptional response elements (TREs) are positioned upstream of a minimal CMV promoter (mCMV) and the pGreenFire luciferase-T2A-GFP co-expression cassette. Throughout the absence of transcriptional activation, the mCMV promoter has negligible train resulting in little- to no- luciferase train or GFP fluorescence (Decide 1). Nonetheless, upon activation of the TREs, similar to in response to the addition of an inducer, the TREs plus the mCMV promoter drive expression of every luciferase and GFP in a dose-dependent development (Decide 1). The result is the flexibleness to quantitatively measure pathway activation using luciferase train or whereas imaging using GFP.
As with all of our pGreenFire 2.zero lentivectors, the GreenFire cassette now consists of crimson firefly luciferase (rFLuc), a T2A co-expression part, and GFP. The swap to rFLuc opens up the potential of performing a dual-spectral luciferase assay and likewise delivers bigger sensitivity for in vivo capabilities than typical luciferase.
Description: ARHGDIA regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Description: The CLCN5 gene encodes the chloride channel Cl-/H+ exchanger ClC-5. This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
