SBI Lentiviral Technologies

SBI Lentiviral Technologies pGF2 mcmv

Overview

Make your private pGreenFire 2.zero reporter vector or use as a detrimental administration

With the pGreenFire 2.zero mCMV Cloning & Unfavorable Administration Lentivector & Virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro), you could benefit from our sturdy pGreenFire 2.zero lentivector know-how to create your private transcriptional response part (TRE) reporter or use pGreenFire 2.zero mCMV as-is as a detrimental administration.

With the pGreenFire 2.zero mCMV Cloning & Unfavorable Administration Lentivector, XhoI and NheI web sites are positioned upstream of a minimal CMV promoter (mCMV) so you could clone in your private TREs. Upon activation, the TREs and mCMV promoter collectively drive co-expression of crimson firefly luciferase and GFP so you could quantitatively measure transcriptional train using every fluorescence and luciferase train.

Alternatively, you should use the vector as-is as a detrimental administration for any enterprise using pGreenFire 2.zero lentivectors (see info below).

What makes our next-gen pGreenFire 2.zero vectors even larger than totally different TRE reporter vectors is the great design, which gives in a constitutive selection cassette for regular cell line period whereas minimizing interference with the upstream TRE. By using a weak/cheap mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and totally arranging the conditional reporter genes, the selection marker is reliably expressed with out compromising conditional expression of rFLuc and GFP.

SBI Lentiviral Applied sciences
SBI Lentiviral Utilized sciences

NEW PRODUCT ALERT!

SBI is labored as much as announce the launch of our subsequent period of pGreenFire signalling pathway reporters! We’ve upgraded these normal lentivectors with a clever design that allows reliable period of regular cell traces and have moreover modified the standard luciferase reporter with crimson firefly luciferase, which opens up the potential of performing a dual-spectral luciferase assay and delivers bigger sensitivity for in vivo capabilities than typical luciferase.

pGreenFire Fundamentals

For many who’re not acquainted with our pGreenFire reporters, every 1.zero and a few.zero share a similar core efficiency—transcriptional response elements (TREs) are positioned upstream of a minimal CMV promoter (mCMV) and the pGreenFire luciferase-T2A-GFP co-expression cassette. Throughout the absence of transcriptional activation, the mCMV promoter has negligible train  resulting in little- to no- luciferase train or GFP fluorescence (Decide 1). Nonetheless, upon activation of the TREs, similar to in response to the addition of an inducer, the TREs plus the mCMV promoter drive expression of every luciferase and GFP in a dose-dependent development (Decide 1). The result is the flexibleness to quantitatively measure pathway activation using luciferase train or whereas imaging using GFP.

As with all of our pGreenFire 2.zero lentivectors, the GreenFire cassette now consists of crimson firefly luciferase (rFLuc), a T2A co-expression part, and GFP. The swap to rFLuc opens up the potential of performing a dual-spectral luciferase assay and likewise delivers bigger sensitivity for in vivo capabilities than typical luciferase.

ARHGDIA Antibody / RHOGDI Antibody

F54788-0.4ML 0.4 ml
EUR 379

Antibody

A1360-500 Ask for price

Anti-Glycolipid Antibody (AGA) Antibody

20-abx004855
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Ly1 Antibody Reactive (LYAR) Antibody

20-abx008109
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Ly1 Antibody Reactive (LYAR) Antibody

20-abx123734
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

Ly1 Antibody Reactive (LYAR) Antibody

20-abx014333
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-400ul 400 ul
EUR 627.6

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-80l 80 µl
EUR 343.2

Anti-Glycolipid Antibody (AGA) Antibody

abx036399-100ug 100 ug
EUR 469.2

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319900
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319901
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319905
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319913
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycolipid Antibody (AGA) Antibody

abx230204-100ug 100 ug
EUR 577.2

Ly1 Antibody Reactive (LYAR) Antibody

20-abx324434
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody

20-abx311665
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody

abx234901-100ug 100 ug
EUR 661.2

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 332.4
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 332.4

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 332.4

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 332.4

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